Biology 2581B Lecture Notes - Lecture 21: Transposase, Polyadenylation, Gamete
Document Summary
Lecture 21: transposable elements and variations in chromosome numbers. Classification: retrotransposons retroposons, transpose via reverse transcription of an rna intermediate, transposons, transpose dna directly without an rna intermediate. Te"s come with their own moving information self-perpetuating. Almost 45% of the human genome is made of te"s. Lines: long interspersed elements, sines: short interspersed elements. In situ hybridization with a probe for copia sequences. 30-50 copies each but often in different positions position of te has shifted relative to genes. Polytene are in the giant glands of drosophila. We can use these for in situ hybridization. Here they used a probe against copia sequences. Different geographic locations = not physically close. We can use te"s to estimate how closely related particular individuals are that come from the same species. Same number of copies, but in different positions. There is lots of time for this te to jump around.