Biology 1001A Lecture Notes - Lecture 5: Dna Replication, Replisome, Metaphase
29 Sep 2016
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Mitosis checkpoint is a metaphase, cell a is in metaphase and is 4c. A distinct 3" and 5" end confers polarity on dna backbones. Daughter strands contain one old backbone, and one new backbone. Dna polymerase adds to a free 3" oh: Every dna polymerase adds basses onto the 3" oh. Orange things: dna polymerase that"s extending 3" ends. Because they are antiparallel, polymerase read 3" to 5" which allows them to add to the. Bottom has to be constantly primed by the rna primers. **know the function of each enzyme/protein at the replication fork** Replisome = all the stuff at the replication fork. Replication bubble arises from two forks created at one origin . Fork on either side of the bubble, going in opposite direction. Dna will be lost with every round of replication due to loss of primer. Cannot be prevented, but can be worked against. Read 3" to 5", rna primer uses u instead of t.
