Biology 1002B Lecture 9: Integrated Metabolism

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Have an intact chlamydomonas cell: mitochondria and chloroplasts in the same cell in chlamydomonas so we want to measure gas exchange, flask of chlamydomonas and a co2 analyzer, to get a measure in the change of. More cells = the rate will go up (more cells fixing carbon) Depending on the photosynthetic system the light response curve will look different, so those lights can be adjusted and will then measure co2 fixation rate. On the y-axis in the dark, what is the co2 fixation rate: below zero. The easiest way to do this is to measure the rate of product formation (p) This diagram is what you end up with. Looks very much like a light response curve the velocity of the reaction, the amount of product being made per unit time: velocity as a function of substrate concentration. Fast when there is low substrate: that is high affinity but low km. There are inhibitors to enzymes working correctly.

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