Biology 1002B Lecture Notes - Lecture 8: Electrochemical Gradient, Chemiosmosis, Pyruvic Acid

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Change in respiration rate (oxygen consumption) in isolated mitochondria by nadh, Adp, uncoupler: mitochondria is added, initially there is no oxygen in the chamber (mitochondria, but when you add mitochondria, the rate of oxygen (the slope) is going downward- less steep. That means that oxygen is being consumed in the chamber: nadh is added, the slope is decreasing- steeper downward- when nadh is added. The rate of oxygen consumption is going up: it is because you have isolated mitochondria. Isolated mitochondria doesn"t have the source of substrate. There is no pyruvate being transported into the mitochondria. Oxygen consumption in etc is independent with atp synthesis, therefore it would not affect the rate of oxygen consumption. Thus, they influence each other: but the rate will go even faster when we add adp. The fact that you already have a huge proton gradient established, limit the overall rate of electron transport.

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