Biology 1002B Lecture Notes - Lecture 18: Agrobacterium Tumefaciens, Mental Floss, Ribosome-Binding Site

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Indp for class 18 03/08/16 (figures 15. 1 15. 4, 15. 11, Overview of cloning dna fragments in a bacterial plasmid. The restriction site for the restriction enzyme ecori, and the generation of a recombinant dna molecule by complementary base-pairing of dna fragments produced by digestion with the same restriction enzyme. Using an expression vector to synthesize a eukaryotic protein. Introduction of genes into mouse embryos using embryonic germ-line cells. Using the ti plasmid of rhizobium radiobacter to produce transgenic plants. Lecture 18 optogenetics i 03/09/16: answer is d. it is an alternatively spliced intron. It is not bicoid mrna because it (the mrna) is made by females in order to pack it in the egg: but those eggs could be males or females once fertilization occurs. Both boys and girls translate the bicoid mrna, but only females will make bicoid mrna. 2nd clicker question: oncomir is a mirna, polya tail is not transcribed.

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