Biology 3316A/B Lecture Notes - Lecture 1: Har Gobind Khorana, Essential Amino Acid, Interactome
20 Feb 2019
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Adv cell bio 3316 lecture 1 cell signalling. Egg shell organizing factor 1 (eof1: malaria is one of the #1 killers in the world. Eof1 is a protein & molecular weight is 93,000 da. If alter this protein, then can disrupt the ability for these mosquitos to propagate: western blot. Looking for levels of protein: need antibodies to detect the proteins (control, eof1, etc. ) Lane 1: need a control to ensure there"s protein in every single lane, maybe use a housekeeping protein, bc it"s expressed no matter what you do to the cell. Take protein (aka eof1) used to make the antibody, from the females, to prove you don"t see in it males bc its female specific. Lane 3: use protein from the male, bc again trying to prove that this is female specific. Lane 4: alter the protein to see what happens.
