Microbiology and Immunology 3100A Lecture Notes - Lecture 11: Epidermal Growth Factor Receptor, Sh2 Domain, Signal Transduction
19 Apr 2020
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Receptor tyrosine kinases (rtk: structure, dimerization, autophosphorylation. Adapter proteins, grb2: ptb, sh2, sh3. Ras and its regulation (gef, gap, sos) Ras/map kinase pathway operation (raf, mek, mapk), egf. That in turn will activate intrinsic kinase activity. Intrinsic kinase activity activates adaptor proteins which leads to activation of ras: adapter proteins are required. Ras acts as a gtpase switch protein to signal further downstream kinases initiate a kinase cascade. Aberrant signaling is at root of many human cancers. A lot more steps in receptor associated kinase activity. Rtk without bound ligand: rtk exists as a monomer and has very low or almost no kinase activity (poorly active protein tyrosine kinase). When a ligand binds to rtk, if there"s two that have their ligand bound, they develop affinity for one another and dimerize. Dimerization allows for trans-autophosphorylation of cytoplasmic domains (each kinase will phosphorylate the other one and become activated).
