BI236 Lecture Notes - Lecture 3: Semiconservative Replication, Dna Replication, Deoxycytidine Triphosphate
Document Summary
Each parent molecule serves as template for synthesis of complementary daughter strand: semi conservative replication. Meselson and stah experiment to prove semiconservative replication. Single origin of replication (ori: special dna sequence. Replication forks: proceeds strands replicated, theta ( ) replication. Replicons: multiple replication units, length of 50-300kb pairs, several thousand per chromosome, origin of replication: special dna sequence in the center. Replications is followed by mitosis and cytokinesis. Dna polymerase: requires small amount of dna as template, elongates dna chain at the 3" end, synthesis only in the 5" to 3; direction, adds datp, dttp, dgtp, and dctp, ppi released to provide energy. Synthesis at the replication fork: leading and lagging strands. Dna ligase joins the okazaki fragments together. Dna polymerase can only add nucelotides to the 3" end of an existing chain. Dna polymerase (iii) adds nucleotides to 3" end of primer. Another dna polymerase (i) replaces rna with dna. Double helix must be unwound at replication fork.
