BI236 Lecture Notes - Lecture 6: Adenine, Poly(A)-Binding Protein, Alternative Splicing

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silvercat239

26 Jan 2018

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Required to bind to its promoter and initiate rna synthesis. Their (cid:374)a(cid:373)es i(cid:374)clude (cid:858)tf(cid:859) (cid:894)tfiia, tfiib etc. (cid:895) General transcription factors in binding rna polymerase ii to dna. Tfiid first binds to tata box in the dna sequence. All others primarily interact with each other. Thus, protein-protein interactions play a crucial role in binding stage of eukaryotic transcription. Preinitiation complex a large complex of proteins including rna polymerase ii. General transcription factor tfiih possesses both helicase activity, that unwinds dna, and a protein kinase activity that catalyzes the phosphorylation of rna polymerase ii. Phosphorylation changes the shape of rna polymerase ii, thereby releasing it from the transcription factors, so that it can initiate rna synthesis at the startpoint. Helicase activity unwind the dna, so that rna polymerase molecule can begin to move. Tfiid - ability to recognize and bind to dna promoter sequences is conferred by one of its subunits, tata-binding protein (tbp)

Related Questions

1. What is the term when the product of a biochemical pathway inhibits the activity of the first enzyme of a pathway?

		feedback inhibition.
		activator inhibition.
		diffusional regulation.
		enzyme activation.

2. Through control of gene expression, prokaryotes respond to changing __________ conditions.

		internal
		protein
		environmental
		genetic

3. A protein that negatively regulates transcription by binding to the operator is known as the

		operon
		repressor
		activator
		inducer

4. During attenutation, when tryptophan levels are high, the ________ stem-loop forms and transcription ________ the trpL gene.

		1-2, ends just past
		3-4, ends just past
		1-2, continues beyond
		3-4, continues beyond

5. The process of turning on the expression of a gene in response to a substance in the environment is called:

		constituation.
		repression.
		induction.
		catabolism.

6. What is a function of some specific transcription factors?

		to bind to the promoter region of the gene to facilitate the proper alignment of the RNA polymerase
		to bind to the centromere to induce meiosis
		to bind to enhancer regions to help regulate gene transcription
		to bind to the operator to recruit sigma factor

7. How does exposing an E.coli cell to glucose affect the regulation of the lac operon via CAP?

		cAMP binds to CAP and transcription is increased.
		cAMP binds to CAP and transcription is decreased.
		cAMP does not bind to CAP and transcription is increased.
		cAMP does not bind to CAP and transcription is decreased.

8. The lac repressor protein works when mutations in the repressor gene and structural genes are:

		in cis or in trans.
		in trans only.
		in cis only.
		in both prokaryotes and eukaryotes.

scarletwombat949

You only need to answer #4! The rest of the questions are there for relevancy.

1. To facilitate your study, you create a GFP tagged version of Tral. Which one of the following is not a step you would perform to accomplish this?

A. PCR the Tral gene from Drosophila genomic DNA.

B. Digest the PCR product and a GFP-containing plasmid.

C. Add the insert and vector together and add DNA ligase.

D. Transform the ligation mixture into Drosophila.

E. All of the above would be performed.

2. For this cloning, we need to include a _______________ promoter in the plasmid.

A. Bacteria

B. Drosophila

C. Neither

D. Both

3. You also speculate that various transcription factors could be regulating Tral expression. One such factor is Snail.

a. If Snail is a transcription factor activating Tral expression, which of the following could potentially be a function of Snail? Select the best answer.

A. Inhibit the binding of RNA polymerase at the Tral promoter.

B. Recruit a histone modifying protein that opens up the chromatin at the Tral gene.

C. Recruit DNA helicase at the origin of replication.

b. You want to determine whether Snail binds to the Tral promoter DNA. Which technique(s) would allow you to determine whether this occurs. Select all that apply.

A. Quantitative RT PCR

B. Chromatin Immunoprecipitation

C. Immunoprecipitation

D. Western blot

E. DNA footprinting

4. Now you want to determine the effect of the loss of Snail on Tral-GFP expression. IfSnail is an activator of transcription, draw/describe the expected results from a:

a. Northern blot

b. Q-RT PCR reaction

The TATA-binding protein (TBP) binds to the TATA box sequence in eukaryotic promoters. What is its function in transcriptional initiation?

It blocks access of RNA polymerase to the promoter, until removed by general transcription factors.

It is the subunit of prokaryotic RNA polymerase that is required to recognize promoters.

It modifies histones so nucleosomes can be removed from DNA for transcription.

It bends and partly unwinds DNA at a promoter.

The genetic code is said to be âdegenerateâ because

there are more codons than amino acids.

there are more amino acids than codons.

different organisms use different codons to encode the same amino acid.

some codons specify more than one amino acid.

Three general mechanisms appear to be responsible for the conversion of proto-oncogenes to oncogenes

overexpression, point mutations, deletions

inversions, translocations, methylation

familial, sporatic, phosphorylation

None of the above account for the conversion of proto-oncogenes into oncogenes

Transcriptional control of genes that acts by regulating the continuation of transcription is called

induction

attenuation

antitermination

negative inducible control

The genetic code is fairly consistent among all organisms. The term used to describe this consistency is

The F, G, and H loci are linked in the order written. There are 30 cM between F and G and 30 cM between G and H. If a plant Ff Gg Hh is testcrossed, what proportion of the progeny will be ff gg hh, assuming no interference?

DNA synthesis is always from 5â to 3â because

replication must be continuous

the strands are antiparallel

primers lack 3â to 5â exonuclease activity

In the ZZ-ZW sex-determination system, if an AaBb female was crossed to an individual of genotype Aa Bb, what is the probability of a female offspring with the two dominant traits given by alleles A and B? Assume A and B are dominant alleles.

BI236 Lecture Notes - Lecture 6: Adenine, Poly(A)-Binding Protein, Alternative Splicing

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