BIOL 2020 Lecture Notes - Lecture 13: Peptide, Ionizing Radiation, Transcription Factor Ii B
29 Apr 2016
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Document Summary
E. coli, rnap binds to a speci c sigma factor to form a polyenzyme. The rst ntp is added (only one that keeps its 5" end; all other ntps lose their beta and gamma phosphates?) The next ntps are added one by one: phosphodiester bond is made, pyrophosphate (ppi; diphosphate) is released, then hydrolized to two inorganic phosphate (pi) molecules. Intrinsic (rho independent: two main parts: inverter repeat or peak sequence and t rich region, t-rich region is re synthesized as a u-rich region on rna. Rna polymerase is often abbreviated as rnap or pol (ex. Each rnap is responsible for transcribing a different class of genes. Major ones to remember: rnap1 transcribes ribosomal rnas, rnap2 transcribes mrnas (protein coding genes, rnap3 transcribes trnas. Much more complex than in prokaryotes, and tend to be more diverse and variable. Promoters consists of the core promoter and additional dna sequences that regulate transcription.
