BIOL 2021 Lecture Notes - Lecture 8: Oligosaccharide, Immunoglobulin Light Chain, Mannose
14 Apr 2017
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Gpi anchor figure 12-52: 1) inserted as transmembrane, 2) cleaved and attached to gpi anchor in lumen of er. Figure 10-24 asymmetry of proteins in pm: orientation maintained during vesicular transport, in er lumen: Figure 12-47b rough er: dolichol = lipid that carries oligosaccharide. Transferred to asn: figure 12-47a n-linked oligosaccharide (nitrogen is attached) Particular amino acid sequence (for recognition and attachment) Figure 12-47a mannose trimming acts as a timer (prev. page: to much trimming -> exported to cytosol, out of er (translocation across membrane) Almost all lipids are made in er membrane: smooth er. Figure 12-54a lipid synthesis enzymes: in er membrane on cytosolic side, new lipids inserted into cytosolic leaflet, scramblase equilibrates. Lipids in 2 leaflets: figure 10-15 asymmetry of lipids in pm. Moved ptd-ser + ptd-etn to cytosolic side. Figure 12-5 look back, now we are looking at green arrows (er) Figure 13-3a roadmap of depth of vesicle traffic: biosynthetic-secretary pathway (red arrows)
