BIOL 2040 Lecture Notes - Lecture 4: Polyacrylamide Gel Electrophoresis, Phenylalanine, Isoleucine
Document Summary
Direct detection of segregation of dna using different methods. This was perhaps the first method used to follow dna segregation directly. Below, these are based upon use of pcr. Snp"s - single nucleotide polymorphism [follow a single nucleotide change: many ways of detecting dna segregation. [rflp (detect restriction fragments by using southern analysis); cleaved amplified polymorphism; Ends up with hundreds of amplifications of just the region of interest. Then put in a restriction enzyme and cut that gene. May see 5 fragments, because the enzyme cuts 5 times. doesn"t matter how many fragments there are, heterozygotes will always be additive. [bands in aa and aa will equal aa: theory of dominance, only see 1 homozygous pattern and 1 heterozygous pattern on the gel. Sscp- single stranded conformational polymorphism: another method of detecting segregation of dna, can detect one nucleotide difference between two alleles, pcr amplify the gene of interest from each genotype.
