BIOL 1100 Lecture Notes - Lecture 6: Apoptosis, Mitosis, In Silico
13 Mar 2017
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Document Summary
Introduction to calcium signaling: the gradient is what makes calcium a great signaling molecule, ec: 1 mm, ic: 100nm, er/mitochondria: storage. Insp3r pathway: high calcium in ec --> low calcium in cytosol --> high calcium stores. Insp3r lets calcium from the er into the cytosol: serca pumps calcium back into the er from the cytosol. Ip3 receptor: found in er throughout the cell (especially purkinje cells in cerebellum) Inhibition is long-lasting --> stay inhibited until get back to low levels of ca: normal curve dependence, positive feedback followed by negative feedback --> essential for oscillation. Frequency can be modified by: hormones (can induce oscillations, drugs, channel modification, co-factors. Regulation by drugs: paclitaxel, anticancer, neurotoxic drug, blocks m --> a transition in mitosis and shrinks tumors, binds to ncs-1 (neuronal calcium sensor 1) In silico docking of paclitaxel to hydrophobic pocket in the middle of ncs-1: nsc-1 is a high affinity, low capacity ca binding protein.
