GEN-3000 Lecture Notes - Lecture 15: Electric Shock, Bacteriophage, Lysogenic Cycle
Document Summary
F factor: a plasmid, the f+ cell has a plasmid, the f- cell has no plasmid, the f factor can integrate itself into the bacterial chromosome, hfr cells behave like f+ cells. They form pili and conjugate with f- cells. The f factor has been integrated into the bacterial dna. Because the f factor is in the bacterial chromosome, some of the bacterial. Dna can get pulled into the recipient cell. The amount depends on the length of time that conjugation takes. This is when homologous recombination takes place. An episome can insert itself into or remove itself from a genome. F": has an f factor, the plasmid contains bacterial genes. Dna on it and a f- cell: a special kind of f" cell. It is rare for the entire chromosome to be transferred during conjugation because it takes about one and a half hours and the bridge between the cells does not stay open for that long.
