BIOSC-101 Lecture Notes - Lecture 11: Cell Adhesion Molecule, Ionic Strength, Histidine
26 Aug 2020
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Document Summary
In cells we can stain parts of the cell in order to visualize different regions of cells. To visualize these cells, we must first culture these cells (grow them in an isolated setting) In order for these cells to be survive with normal function, we must ensure that we have certain components in place : They need a set temperature (in incubators) They need a set ph value (using buffers we can maintain this) The culture media must be nutrient rich. They need to have a specific container like a dish or a flask, so they have a. They need very sterile conditions to work in (with bio-safety cabinets and. They need antibiotics to ward off diseases. Histidine, isoleucine, leucine, lysine, methionine, phenylalanine, surface to adhere to sterilizing agents) The culture media must have specific nutrients threonine, tryptophan and valine. 3 specialty amino acids (which are made by specific cells) Serum (fluids remaining after blood has been allowed to clot)
