PCB 3063 Lecture Notes - Lecture 39: Lac Operon, Adenylyl Cyclase, Allolactose
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What are the control regions of lac operon and their function? laci (i gene): codes for the repressor protein lacp (promotor): binding site for rna polymerase laco (operator): binding site for the repressor protein. The repressor binds to the laco site, preventing the binding of the rna polymerase. Allolactose binds to the repressor, preventing it from binding to the laco. What is the effect of glucose levels on cap activity: low glucose levels induces adenylate cyclase activity (which catalyzes the hydrolysis of atp into camp. + pp: cytoplasmic levels of camp (catabolite) increases, camp activates cap, activated cap facilitates the binding of rna polymerase to the lac promoter. What is necessary to bind rna polymerase to promoter in lac operon when glucose levels are low or. 1. camp levels decrease, cap is inactivated: rna polymerase does not bind to promotor, operon is off. Present- structural genes are turned off by trp itself.