BIOL 358 Lecture Notes - Lecture 2: Basal Body, Bacterial Capsule, Surface 3
Document Summary
Lecture 2. 1 cellular basics and external prokaryotic structures and functions. Process of life: growth (ice size, reproduction (inc in number, responsiveness (react to environment, metabolism (control chemical reactions) Prokaryotes: lack nucleus, lack various internal structures bound with phospholipid membranes, are small (~1. 0 um diameter, have simple structure, include bacteria and archaea. Check for understanding: a man was infected with the gram-negative bacteria e. coli and given penicillin. The antibiotic did not impair e. coli growth. All are possible reasons why it did not work except: penicillin dose was too low, penicillin could not cross the outer membrane, penicillin removed the lps from the outer membrane. 2. 4 microscopy: observable objects with different microscopes. Principles of staining: staining increases contrast and resolution by coloring specimens with stains/dyes, smear of microorganisms (thin film) made prior to staining, microbiological stains contain chromophore, acidic dyes stain alkaline structures, basic dyes stain acidic structures.
