BIOM-360 Lecture Notes - Lecture 6: Agaricus Bisporus, Media General, Glucose
2 May 2018
School
Department
Course
Professor
Generally, not expected to know things that Dr. Branco has not talked about.
Read parts of book that she has covered.
Pressure
Microbes on land and water surface → 1 atm.
Some bac and arch live in hydrostatic deep sea conditions (barotolerant, barophiles).
Microbial environments are complex, dynamic, oligotrophic (lacking nutrients).
Such conditions may expose a microorganism to overlapping environmental gradients.
Biofilms
Most microbes grow attached to surfaces.
Attached microbes are members of comles, slime-enclosed communities called biofilms
.
Ubiquitous in nature.
Can be formed on any surface.
Culture Media (Food)
Culture medium is used to grow, transport, and store microorganisms. Agar is an example.
PDA- Potato Dextrose Agar.
Can be solid or liquid.
Must contain all the nutrients required by the organism for growth (major, minor, trace elements, etc).
Types of Media
General purpose media
Support growth of many microorganisms.
Ex: Tryptic soy agar.
Enriched media
General purpose supplemented by blood or other special nutrients.
Ex: Blood agar.
Selective media
Allows growth of specific organisms and inhibits others.
Ex: MacConkey agar tests for Gram negative bacteria.
Differential media
Allows one to distinguish between groups based on characteristics.
Differentiate close relatives.
Ex: Hemolytic versus non-hemolytic bacteria.
Isolation of Pure Cultures
Portobello mushroom exercise.
Groups of cells arising from a single cell.
Allows for the study of a single type of microorganism.
Techniques used to isolate pure cultures: Spread plate, streak plate, and pour plate.
The Growth Curve
Observed when unicellular microorganisms are cultivated in batch culture (liquid).
Has four distinct phases.
Document Summary
Generally, not expected to know things that dr. branco has not talked about. Read parts of book that she has covered. Microbes on land and water surface 1 atm. Some bac and arch live in hydrostatic deep sea conditions (barotolerant, barophiles). Microbial environments are complex, dynamic, oligotrophic (lacking nutrients). Such conditions may expose a microorganism to overlapping environmental gradients. Attached microbes are members of comles, slime-enclosed communities called biofilms . Culture medium is used to grow, transport, and store microorganisms. Must contain all the nutrients required by the organism for growth (major, minor, trace elements, etc). General purpose supplemented by blood or other special nutrients. Allows growth of specific organisms and inhibits others. Ex: macconkey agar tests for gram negative bacteria. Allows one to distinguish between groups based on characteristics. Groups of cells arising from a single cell. Allows for the study of a single type of microorganism.
