RLT 48500 Lecture Notes - Lecture 5: Ninhydrin, Phenylalanine, Tripeptide
2 Oct 2016
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Note: entries in bold letters are the aas primarily destroyed. Hydrolysis: bond cleavage of labile bonds simultaneous with the addition of water, needed to break amide bonds in intact proteins to produce amino acids. Acid hydrolysis catalyzed by strong acids such as h2so4, Base/alkaline hydrolysis uses strong bases such as a(oh)2, naoh, koh, etc. (15 psi / 5hrs: total hydrolysis, trp is not destroyed, promotes racemization, thr and cys are lost, arg is destroyed and converted to urea & ornithine. Biuret test general test for intact proteins and protein hydrolyzates (at least a tripeptide: named after the compound biuret, reagents: cuso4 sol"n + dilute. Naoh: (+) result: formation of pink to violet to blue color, principle: complexation of cu+2 w amide n atoms, no rxn w dipeptides, urea, coagulated proteins and amino acids (except ser & thr) Some amines, such as aniline, yield orange to red colors, w/c is a negative test.
