BIOL 1121 Lecture 7: Microbial Growth
15 Mar 2017
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Document Summary
Robert koch (1843-1910: studied disease-causing bacteria (tb, methods of growing bacteria. Karina da rosa: solid media to allow single bacteria to grow colonies, solidified liquid nutrient media : gelatin, major issue : melting temperature and digestibility, wife suggested usage of agar, microbial habitats i. ii. Prokaryotes can be found in severe conditions: ocean depths, volcanic vents, polar regions. Prokaryotic cells multiply by binary fission: exponential growth : doubles during each division, generation time : time it takes for the population to double ii. Growth calculations: n t = n o x 2 n, n t - number of cells in a population at time t , n o - initial number of cells c. 2 n - number of generations at that point: rapid generation time with optimal conditions can yield huge populations quickly, depends on species and growth conditions iii. Growth in labs: prokaryotes are grown on agar plates, in tubes or flasks and broth.
