BIOL 2301 Lecture Notes - Lecture 3: Klf4, Two-Dimensional Gel Electrophoresis, Rna-Seq
19 Jun 2016
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Document Summary
Recombinant dna: the joining together of two or more deoxyribonucleic acid molecules, often from different organisms. The resulting molecule is then usually incorporated into a living cell, where it replicates. Insert dna fragment into the plasmid = recombinant dna. Sticky ends of fragment and plasmid match because same restriction enzyme was used for cleavage. Transformation of bacterium and selection of a cell containing the plasmid. Produce drugs/pharmaceuticals: insulin, growth hormone, blood clotting factor viii. Making genetically modified organisms (see gmo lecture) Knocking things out with rnai: for agriculture. Synthetic biology: making cells do stuff, making new organisms. In response to high levels of glucose in the blood, the insulin-producing cells in the pancreas secrete the hormone insulin. Type i diabetes occurs when these cells are destroyed by the body"s own immune system. Insulin: a small protein containing of 51 amino acids, synthesized and stored within the pancreas. The protein consists of 2 chains, a and b.
