BIOSC 0160 Lecture Notes - Lecture 12: Acetyl Group, Intron, Regulatory Sequence
25 Oct 2017
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Document Summary
Initiation complex: regulatory transcription factors are responsible for expression of only particular genes. Also binds to silencers or enhancers: basal (general) transcription factors: interact with promoter (tata box). Are around all the time and are required for: mediator complex: does not bind to the dna, binds to the rtf and btf and brings them together. Chromatin remodeling: methyation, ch3, occurs on cytosine residues, methylated c"s bind to g"s as normal, anytime dna is heavily methylated, we get suppression of the expression of particular genes. Genes are inactive until methyl groups are removed: methyl groups are reversible: can take away or add, epigenetic changes. Regulatory sequences: upstream of promoter, there is a proximal promoter region. Regulatory transcription factors bind to the promoter proximal region: +1 site is not in protein (mrna), promoter is not in rna. Tonegawa experiment: started with a human antibody producing gene, took enzymes, and used those enzymes to take out different parts of introns.
