01:447:380 Lecture Notes - Lecture 8: Phenylalanine, Wild Type, Thiamine
Document Summary
1: phototrophs: bacteria wild-type for all growth requirements; can survive. In liquid culture or on solid media in petri dish on . Genotypes (neither strains grow on minimal media) Y24: phe- cys- bio- thr+ leu+ thi+ Y10: phe+ cys+ bio+ thr- leu- thi- If there is growth = must be phototrophs/wild-type. Mix of mutant strains growth colonies formed. Expected genotype: wildtype, because it"s growing on minimal medium. Gene transfer in prokaryotes: f (fertility) factor. Rarely gets across pilus: entire f , f factor can pop back out of bacterial chromosome: sometimes takes nearby chromosomal genes with it; plasmid now called . F": conjugation between f" and f- creates contains 2 copies of a few genes, f": has some piece of bacterial chromosome within it. Gene linkage in prokaryotes (only applicable with f-factors???) (f+) bacteria can donate f+ (f-) bacteria can accept. Interrupted mating experiments ( kitchen blender experiments), by ellie wollman.
