01:447:484 Lecture Notes - Lecture 8: Explicit Memory, Synaptic Plasticity, Calcineurin
18 Jun 2016
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Pronuclei injection is done by taking a fertilized and injecting dna into egg. Up to 50 copies can be inserted in one place. Faster, shows more expression, and randomness might be useful. Positional effect: the nearby surrounding sequences will affect the sequence in affect. Control of transgene expression and conditional knockout in mice: Conditional knockout: make the knockout only in certain places. This is done using promoters that are only expressed in certain cell types. Wiki def: conditional gene knockout is a technique used to eliminate a specific gene in a certain tissue, such as the liver. This technique is useful to study the role of individual genes in living organisms. It differs from traditional gene knockout because it targets specific genes at specific times rather than randomly knocking out genes. Using the conditional gene knockout technique eliminates many of the side effects from traditional gene knockout.
