01:694:301 Lecture Notes - Lecture 29: Pribnow Box, Transcription Bubble, Phosphodiester Bond

• School: Rutgers University
• Department: Molecular Biology and Biochemistry
• Course: 01:694:301
• Professor: F Deis

Document Summary

3 stages of transcription: promoter binding and initiation, elongation, and termination: rna polymerases catalyze the synthesis of rna and have multiple functions: 1) they search for dna initiation sites (promoter sites) 2) they unwind short stretch of double helical dna ss dna templates from which sequences can be read. 3) they select correct rntp and catalyze formation of phosphodiester bond; it"s completely processive. 4) they detect termination signals where transcript ends. In termination, bond formation stops, rna-dna dissociate, unwound dna rewinds, and rna pol releases dna: the transcribed regions of dna templates contain stop signals, termination of prokaryotic transcription occurs 2 main ways: After this sequence, rna pol pauses and rna-dna hybrid helix is unstable (u-a base pairs are weakest) This allows nascent rna to dissociate from dna template and enzyme: rho ( ) protein. Hexameric hydrolyzes atp in the presences of ss rna but not presence of dna or rna duplex.