BIOL 115 Lecture Notes - Lecture 27: Recombinant Dna, Restriction Enzyme, Ecori
11 May 2020
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Biol 115 lectures 27-31 molecular genetics and biotechnology: molecular genetic analysis and biotechnology, molecular biology techniques have dramatically altered how genes are studied, used in every field of biology. Major innovations in molecular biology: development of recombinant dna technology, invention of pcr, rapid sequencing of dna, crispr-cas9. Palindromic repeats: avoids expense and time of creating zfns or. Crispr associated sequences: grnas can base pair with virus they originated in and target it for endonuclear destruction, bacterial cells have memory of previous infections, can co-opt this system to work in eukaryotic cells, uses crispr-cas9 system from. Stretococcus pyogenes: can modify to include any target sequence from any cell type, if cell can express both, it will cut its own. Selectable and screenable markers: markers tell which cells have incorporated recombinant plasmid, plasmid has markers, bacterial strain has neither, selectable markers antibiotic resistance, screenable markers lacz expression.
