BSC 315 Lecture Notes - Lecture 15: Messenger Rna, Stop Codon, Peptide Bond

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Gene expression, the genetic code and translation. When protein is the final product: dna (transcription, primary transcript (rna processing; capping, polyadenylation, splicing, messenger rna (translation, protein. When rna is the final product: dna (transcription, primary transcript (processing, rna. Amino acid structure: amino: -nh2, carboxyl: -cooh, central (alpha) carbon: bonds to h and r. 20 different r groups = 20 different amino acids. Peptide bond: covalent bond between amino and carboxyl groups in adjacent amino acids. Amino acids differ only in r group. Properties of proteins are determined by properties of r groups. Primary structure: the sequence of amino acids, e. g. , met-ala-thr-phe . Each protein (aka polypeptide) composed of 10s to 1000s of amino acids. Proteins have polarity: amino and carboxyl ends (n and c termini) Proteins fold into three-dimensional shapes, determined by chemical interactions among. Tertiary structures = larger scale 3d structures: globular proteins, fibrous proteins. Examples: lens crystalline = homodimer, hemoglobin = tetramer (2 alpha-globins & 2 beta-globins)

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