BSC 315 Lecture Notes - Lecture 23: Intellectual Disability, Lactase, Rna Splicing
28 Mar 2017
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Why lacz: b-gal not preset in most eukaryotic cells, synthetic substrate x-gal makes detection easy, green fluorescent protein (gfp, can be detected in living cells, insert each hybrid dna construct into genome of the experimental organism. Assay for b-gal experiment: result: some constructs show b-gal expression while others do not, conclusions: regions do not contain enhancers (no expression of reporter gene). Region contains an enhancer that acts in positive regulation and drives expression in the wing: reporter gene expression driven by specific enhancers, lacz reporter gene expression in drosophilia and mouse embryos. Dna methylation and imprinting: verte(cid:271)rate dna (cid:373)ay (cid:271)e (cid:373)odified (cid:271)y c (cid:373)ethylatio(cid:374), usually at 5"-cg-3" sequences. 2: controlled by imprinting control regions (icrs, omit mechanism: pg. 560: imprinting: inactivation of genes in a gamete, some genes are imprinted (inactivated) through methylation in maternal gametes, others in paternal gametes, example: angelman syndrome.
