BIO 201 Lecture Notes - Lecture 8: Endoplasmic Reticulum, Secretion, Nuclear Membrane
6 Sep 2018
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Topological relationship between rer, ser, and nuclear envelope. Not interconnected with er, but similar structure. Divided into cis -, medial, and trans- stacks. Model cell to answer question: pancreatic acinar cells (produce and secrete copious amounts of digestive enzymes) Approach: label newly-synthesized proteins with radioactive isotopes, and watch the labeled protein as it makes its way to be secreted. Pulse-chase experiments allow for the tracking of molecules in a cell over time. During the chase phase, cells are provided with a large amount of nonradioactive material, so they are not labeled. First step in the pc experiment = incubating cells with a labeled molecule for a short time. Premise: h^3 leucine is taken up into newly-synthesized proteins. Labeled protein should be found in different organelles at regular time intervals after labeling. After phagocytosis, organelles will go to lysosome. 1-3 minutes chase: silver grains mostly in rer. 10-20 minutes chase: silver grains in rer and golgi.
