MIC 301 Lecture Notes - Lecture 2: Noncoding Dna, Dna Replication, Green Fluorescent Protein

• School: University of Miami
• Department: Microbiology and Immunology
• Course: MIC 301
• Professor: Williams Roger

Document Summary

Dna may aggregate to form a nucleoid. May also have small self-replicating dna molecules called plasmids. Different dna from chromosome: bacteria pack dna into nucleoids, supercoiled dna into loops which are anchored by histone proteins. 9 dna replication: initiation (oric) elongation termination (ter, each phase uses a lot of different proteins. 10: begins at single origin (oric, 2 replication forks that go around circle in opposite directions. 17: plasmids replicate on their own, usually don"t carry essential genes, introducing plasmids into dna called transformation (horizontal inheritance) 18 plasmid replication: each plasmid has an ori (origin of replication, ori dictates many things: Mechanism of replication = rolling circle or bidirectorial. Plasmid incompatibility group = plasmids with closely related ori"s interfere with each other"s replication and cannot coexist inside the same cell. Copy number = some plasmids have lots of copies and others much lower at 1-2 per cell. Single origin and in two directions at same time.