MCB 2610 Lecture 1: Lab_ Distribution of microorganisms and Isolation of bacteria
13 May 2021
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Plate 2 bench top no wescodyne saline then benchtop. Swab in pbs buffer swab in agar plate on all angles. Always incubate upsidedown air keep it open130min then incubate. Plate 3 wescodyne clean the bench top end labwith this always start. Repeat all the same steps as above plate 4 faucet handle no wescodyne. Control plate never opened to confirm clean media. 0 colonies write nia in lab manual form margin elevation keep plate empty. Form what does it look like shape circular spindle. Elevation how far above the agar is colony growing plate. Note the number of colonies the colony described. Can use plate or culture lintesttube as a media broth. Draw a t behind plate for 7 streak sample name. Let the loop cool burn your loop to sterilize. 3 agitate shake the tube flame thetopof tube don"t let loop touch the sides dip in the culture on the plate.
