PNB 2264 Lecture Notes - Lecture 2: Haematoxylin, Eosin, Microscope Slide
17 Dec 2018
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Histology - the study of the microscopic anatomy of cells and tissues. Immunohistochemistry histology - the process of selectively imaging cellular morphology in a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. Prepare the tissue for viewing: fixation and embedding. Crosslinking using chemical crosslinking agents to preserve the organization of protein and membrane structure. Embedded in resin or plastic and attached to the arm of a microtome: sectioning. Thin sections are cut and assembled to a microscope slide: staining. Hematoxylin and eosin (dyes) are added to enhance contrast. Hematoxylin - blue, basic, positive, binds to dna and rna (basophilic, acidic, negative) Eosin - red, acidic, negative, binds to lysine, arginine (amino acid side. Covers or lines a body surface or cavity. Ex) skin, walls of body cavities, linings of gastrointestinal and urinary tracts. Barrier that substances must pass to enter/exit the body. Single layers (simple) but can be multilayered (stratified)
