MCDB 134 Lecture Notes - Lecture 15: Tissue Tropism, Reoviridae, Serotype
15 Nov 2017
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+/- dsdna genome -> transcripted by v. a. p. (lamda3- pol. ; lamda2 -> guauyl tissue; lamda1: helicas) +mrna -> replication (protein synthesis dependent)-> +/- dsrna (morphogenesis) Fells see that different serotype gives different virulence. To isolate reassortant and see which gene is responsible for the reaction-> mix. The pathogenicity is governed by more than 1 gene. For type 3, m1c is degraded-> virus is unstable. Governed by s1 gene (in particular, the sigma 1 gene) Capacity of virus to inhibit post-translational synthesis/ protein synthesis. S4 gene, in particular sigma 3 that blocks pkr ( which in turns differentiate primary and secondary structure) Has a triple capsid shell (unlike reovirus, which has double capsid) dsrna. Protein nsp4 (product of segment 10) segment 10=transmembrane glycoprotein: viral entertoxin (toxin that is secreted to intestines) 1st virus discovered to be a viral entertoxin. Ca2+ activation mobilization, cl- secretion---> loss of cl- with activation of glycoprotein.
