MCDB 134 Lecture Notes - Lecture 20: Group-Specific Antigen, Pseudoknot, Fusion Protein
15 Nov 2017
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Translation of full-length rna prgag protein and prgag-pol fusion protein. 95% just makes prgag and make gag protein. Gag is a protease and makes capsid protein and nucleocapsid protein so need more. 5% of the time has a frameshift and make prgag-pol. Have a ribosome frameshift pol slips back 1 nucleotide-> different reading frame by bypassing the 1st termination sequence for gag. Pseudoknot: causes the ribosome to frame shift before it gets to the. Only 5% because pol is an enzyme and doesn"t need much of it. Gag and pol in the same frame but termination is suppressed. Cleavage of pr180 generate 95k and polymerase beta, which get cleaved again into a polymerase alpha, which acts as dna polymerase and has. *****in sum, full-length translation make structure protein (pol and gag) and. Translation of spliced rna becomes prenv (glycoprotein) and onc (pp60src) Change in structure of particle causes to be more infectious after release from host cell.
