MCDB 134 Lecture Notes - Lecture 8: Vero Cell, Rhabdoviridae, Animal Virus
15 Nov 2017
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Audio recording started: 10:02 am wednesday, april 19, 2017. Audio recording started: 10:05 am wednesday, april 19, 2017. Currently used vaccine in us against poliovirus is an inactivated trivalent vaccine against 3 serotypes of virus grown in vero cells in culture. We"re approaching global irradiation by national immunization days. China: ~1/4 of world"s children were immunized in a single week in 1992. In 2008, down to 1625 cases; about half of them are in nigera, pak, afg. In 2014, down to 359 cases; most of them are in pak (>300 cases) Antibody targets against vp1 generally, so receptor on virion: vp1 can"t bind. Major one is g protein and m, matrix protein. Negative ssrna: linear and not infectious when isolated. Single cycle time is 6-8 hours with high yield; can get 1000-5000. Virus ends up in endosome and fuses with it. The nc is released into the cytoplasm (contains -ssrna genome, protein l,
