MCDB 134 Lecture Notes - Lecture 5: Differential Centrifugation, Ammonium Sulfate, Reoviridae
15 Nov 2017
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Alternating cycle of high and low g force. Fractionation of particle depends on shape and size of particle (but not it"s density) Viral proteins are more resistant to solvents than cellular protein. Key: find the right solvent for right virus. Ammonium sulfate causes the precipitation of protein and virion. Gel filtration techniques: resins that fractionate based on size. Ion exchange chromatography: fractionate based on charge on the surface of the protein. Organic solvent centrifugation differential centrifugation pellet virions pellet supernatant again velocity centrifugation (based on size and shape) equilibrium centrifugation based on density. Key: receptor concentration dependent on both virus and host da/dt=k[virus][cell] Need electrostatic complementarity (at ph 7, there is a net (-)) Subunit integration (alpha 2 beta 1 and alpha 5 beta p) Compound of capsule is usually the attachment protein. The attachment protein is sigma 1 with 36 molecules. With vesicular stomatitis virus (enveloped, bullet shaped virion) Polio virus (replicate in human but not mouse cells)
