MCDB 134 Lecture Notes - Lecture 16: Mhc Class I, Sv40, Caveolin
15 Nov 2017
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Document Summary
Attachment is to cytoplasmic membrane penetration for endocytosis. Not through clathrin pits, but through caveolin vesicles. Result in viral chromatin dsdna+ 4 cell histones+ vp1 (nls; bind to viral. To release nucleoprotein complex, which is then transported by microtubule motors to nucleus of infected cell. Importin: alpha/beta protein: facilitate entry of structure into nucleoplasm. Transcription and translation (early) early gene expression (prior to dna replication in infected cell) For sv40, they are t and t antigen. Late gene expression, typically encode structural protein is very slow (30-40 hours) at replication. Genome has 1930* ~110 amino acid= 200,000 protein (half of it are expressed early) When look at map of virus with circular genome. At 12 o"clock, is ori at ~0. 67map units=start of ori if circle goes from 0-1, 0/5243 nucleotides around circle. *at 12 o"clock, ecori could be at 12 and at 0/1 for map units.
