MCDB 134 Lecture Notes - Lecture 4: Eclipse Phase, Contact Inhibition, Western Blot
15 Nov 2017
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Virus doesn"t kill the host, but changes cell growth properties (loss of contact inhibition) Area of infection: foci- central site in which a disease localizes or develops. The number of foci is proportional to the concentration of the virus. Unit: focus forming unit/ ml of sample& ffu/ml. Infect cells with viruses such that every single cell will be infected- use moi of. 10, which will result in 98% probability of infection per cell. Determining in a single cycle of growth, what"s the maximum yield of virus is a. b. c. d. Start with adding virus to culture cell (known # of cell) with m. o. i. (multiplicity of infection- how much infectious viruses used per cell to initiate infection). Remove inoc (any virus that isn"t attach to the cell) Increase incubation time, increase amount of virus produced until you reaches max. Now know plaque forming unit, and number of starting cell -> can calculate single cycle yield.
