MCDB 134 Lecture Notes - Lecture 17: Large Tumor Antigen, Nuclear Localization Sequence, Papilloma
15 Nov 2017
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Remove intron to bring together 2 different exons. Intron usually start with gu and ends with ag. Spliced rna be transported from nucleus to cytoplasm. Can delete small t antigen without affecting large t-> small t antigen expression isn"t required large tumor antigen t is required in permissive cell. Function of binding large t antigen to host cell. ~95% nuclear (targeted back to nucleus). led to the discovery of the nls (nuclear localization signal) pro-pro-lys-lys-lys-arg-lys-val (1st nls) *if one of the lys is changed to thr, then the protein is targeted to the cytoplasm instead of nuclear. Biochemical activity binds dna near ori (3 sites where t antigen binds) highest affinity at site 1, then site 2, lastly site 3. T antigen: when t binds to p53, it inhibits p53"s activity. Machinery is cellular occurs in the nucleus of the infected cell. initiation=at ori (single, unique site) site ii of dna replication is bi-directional.
