BICD 100 Lecture Notes - Lecture 18: Lac Operon, Dosage Compensation, Microrna
29 Oct 2016
School
Department
Course
Professor
Document Summary
Lnc rnas-long non-coding rnas: case-study: dosage compensation. Easier to sequence rna than dna (single standed) 2 homo mutants cross epistasis test: found: lin-14(lf); lin4(lf) mutants are precocious. Same phenotype out as lin14, lin-14 is epistatic to lin4. Lin4 encodes no protein, only an rna. Lin14 rna is always present, but protein decreases through larval dev. Lin 4 required for lin 14 decrease. Lin-4 rna potentially could base pair with lin-14 3"utr. Lof in coding seq and gof in 3"utr: experiments to prove idea of suffiency if lin4 is sitting on lin14utr lacz decreases through larval dev lin4 required for lacz decrease. Used lac-z::lin-143"utr reporter transgene to test model. Regulate translation and mrna translation how to prove: Finding: double stranded rna, not single strand is most effective: also affect progeny, not stochastic (greater than 1:1, super important opens up field of rnai. 2 types of rna molecules involved in rna induced gene silencing:
