BIMM 100 Lecture Notes - Lecture 1: Nucleotide Excision Repair, Dna Polymerase Iii Holoenzyme, Dna Polymerase I
8 Jun 2020
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Document Summary
Repair mechanisms: a-form: predominates in higher salt. Increase dna replication fidelity: b-form: most in this form. Allows dna binding proteins, promoters, etc. to bind during trpn. Alpha helix: z-form: important in gene expression. 5" 3": dna pol i adds bases 5" 3" using pa, dna ligase: forms phosphodiester bonds, base excision repair (ber, nucleotide excision repair (ner, mismatch repair, homologous recombination (hr, non homologous end joining (nhej) Cancer therapeutics: cisplatin, binds to dna like a dna adduct, activates ner, best for xeroderma pigmentosum, parp inhibitors, attract repair proteins (esp ber) Inhibit parp double strand breaks more hr (if defective hr then cell gets. Nhej: best for brca1 and brca2, termination, replication fork merges. 5-15kb consisting of ttaggg repeats protects chromosomal dna during replication and protects dna from nuclease attack. Dna) and rna template inside the protein: adult stem cells and germ cells have higher level than other adult cells.
