In class work: write the codons that are needed to make oxytocin. Include any additional codons that are needed besides those encoding your polypeptide (include those in your answer, also include chemical orientation) Is there only one nucleotide sequence that could produce your protein: draw and label rna molecule that could produce this polypeptide. Label all the required regions: can this gene produce more than one protein sequence. Explain why: draw and label the dna region that could produce this polypeptide. The nucleic acid strand(s) with unknown sequence can be depicted as a line. Polysaccharides or complex carbs are polymers of monosaccharide monomers. We do not have enzymes that will break down cellulose. Starch and glycogen are easily hydrolyzed because they have a-glycosidic linkages. Glycogen is hydrolyzed by the enzyme phosphorylase. They can break down glycogen to provide glucose. Amylases play a key role in carb digestion. Cellulose is not hydrolyzed by animals due to the different linkage.

Related Questions

The wild type "puffy" protein in yeast is exactly 103 amin acids long. The amino-terminal portion of the wild type "puffy" protein has the following amino acid sequence (NH2-met-trp-his-tyr-met-gly-cys-tyr-pro-leu-lys-...) B) A mutant yeast cell (Mutant #1) was found that does not synthesize the "puffy" protein. Instead this mutant cell synthesizes a new peptide that is related to "puffy" but this new peptide is only 6 amino acids long and is not found in wild type cells. This peptide has the amino acid sequence (NH2-met-typ-his-tyr-met-gly-COOH. A) what is the specific molecular basis of the mutation in Mutant 1(what is the specific change in the DNA that caused the synthesis of this mutant protein)? Show how you reached your conclusion. Please include the DNA sequence before and after the mutation

Which of the following is NOT a direct consequence of the fact that DNA polymerase can only build onto the end of an existing nucleic acid?

Question 6 options:

	DNA replication requires primers
	Bits of DNA are lost from the ends of your chromosomes in each successive round of replication
	RNA is involved in DNA replication
	The enzyme "primase" is needed for DNA replication
	There are multiple origins of replication on each eukaryotic chromosome

How does a nucleotide sequence code for a protein in nearly all living organisms?

Question 7 options:

	a sequence of 3 nucleotides can code for one of 64 different amino acids
	a sequence of 2 nucleotides can code for one of 64 different amino acids
	a sequence of 2 nucleotides can code for one of 20 different amino acids
	a sequence of 3 nucleotides can code for one of 20 different amino acids
	a sequence of 3 nucleotides can code for 3 different amino acids

Fill in the blanks. To make one strand of DNA, _________ monomers are linked together in a chain, forming a polymer we call a(n) ________ .

Question 8 options:

	nitrogenous bases, hydrogen bond
	nucleotide, nucleic acid
	amino acid, nucleic acid
	nucleotide, nitrogenous base
	amino acid, protein

Which of the following was NOT part of the ideas that Darwin used in his book On the Origin of Species?

Question 9 options:

	organisms vary in characteristics that affect survival and reproduction
	variation in traits is often heritable
	descent with modification explains life's unity and life's diversity
	organisms inherit characteristics from their parents that the parents acquired in their own lifetimes
	natural selection is a cause of adaptive evolution

Here is a hypothetical mRNA sequence:

5' G G A U G C U A G U A U G A 3'

What is the amino acid sequence encoded by this mRNA?

Question 10 options:

	Met-Ile-Val
	Asp-Ala-Ser-Met
	Met-Leu-Val
	Ser-Met-Ile-Val
	Gly-Cys

I need help with Part B. I believe that part A is correct.

a) You also wish to synthesize a degenerate oligonucleotide primer that could be used eventually to clone DNA that encodes your mutant protein. Design this degenerate oligo, keeping in mind the rules we talked about in class. More information about designing degenerate primers and primers in general are located on the following page.

Write out the sequence of this oligo here based on this amino acid sequence (Met Ala Pro Met Val Ala Glu):

My answer (Is it correct?)

I got:

5â AUG - (GCU/C/A/G) - (CCU/C/A/G) - (AUG) - (GUU/C/A/G) - (GCU/C/A/G) - (GAA/G) â3

3â UAC - (CGA/G/U/C) - (GGA/G/U/C) - (UAC) - (CAA/G/U/C) - (CGA/G/U/C) - (CUU/C) 5â

______________________________________________________________________________

(What I actually need help with and have no idea where to begin. Please help me)

b) If you synthesized the above oligonucleotide primer, how many different oligos would be present in the mixture?

Follow these rules Modified from the Thermo Fisher web site:

Designing degenerate primers:

Write out your amino acid sequence, label amino and carboxy termini

For each amino acid, use the genetic code to predict the various nucleic acid codons that can encode this amino acid. For all but Met, you should have redundancy present.

Now you need think about 5â and 3â considerations, and keep in mind that DNA within genes is double-stranded. It is helpful to keep in mind that the 5â end of a gene corresponds to the amino terminus of a protein. Using 5â and 3â labels write out a single strand DNA sequence corresponding to the your amino seq of interest. You may choose to start with only possible codon for each amino acid, or you can do all possibilities using the notation I showed you in class to account for the wobble position of codons.

Now make your DNA double stranded by filling in the opposite DNA strand; label the 5â and 3â ends of this second strand and make sure you have an anti-parallel arrangement of DNA strands.

If you havenât already, now consider the redundancy present on both strands, and make sure you have indicated sequences that account for all possible redundancies.

Realize that when a researched synthesizes a degenerate oligo that the final synthesis contains oligos with every substitution possible. The amount of degeneracy is defined by the number of different primer combinations in the mix. You can determine the degree of degeneracy by multiplying the number of changes present at each position together. If a position has no degeneracy then you multiple by 1 for that position.

Keep in mind that the trade-off between primer specificity and efficiency can be modified by altering the degeneracy of the primer. For example, the more degenerate the primers, the less specific annealing will be; however, decreased degeneracy will allow more potential to identify unknown variants. Anything over 1024 fold degeneracy is at the upper limit of potential usefulness.

BISC207 Lecture Notes - Lecture 12: Choline, Starch, Biological Membrane

BISC207 Full Course Notes

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