MEDT270 Lecture Notes - Lecture 1: Phagocytosis, Cell Envelope, India Ink
3 Jun 2021
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Most bacterial cells are 1-5 micrometers (um) in length. Meter -> millimeter -> micrometer -> nanometer (increase by 1000 folds each time you go down) Refraction- bending of light as it passes from one medium to another. Focal point- a point where the parallel rays of light focus. Condenser and immersion oil in the light microscope. Magnifications- folds increase in the apparent size of the specimen being observed. Compound microscope comprises two or more magnifying lenses (ocular and objective) Total magnification= magnification of ocular multiplied by one of the objective lenses. Resolution- ability to reveal fine detail or two points distinctly. Reported by the smallest distance between two closely-spaced points that can be viewed clearly. Smaller number the resolution is, the better quality of the microscope. Four types of light microscopes used in clinical microbiology labs. Resolution= ability of a lens to separate or distinguish small objects that are close together.
