BSC 2010 Lecture Notes - Lecture 14: Inverted Repeat, Allolactose, Polyadenylation
9 Mar 2016
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Document Summary
Transposable elements (tes: the simplest tes consist of. A gene that encodes an enzyme or excision and insertion of the te sequence (transposase in dna tes) A recognition sequence that the enzyme recognize as the boundary of the te. Transposase recognizes boundaries of te inverted repeats and cuts the te out of the donor site. T"ase cleaves chromosome at target site via endonuclease. Transposon is joined to the single-stranded ends at the target site. Gaps are filled in by dna polymerase i and dna ligase. Result is a new copy (copy and paste) or moved copy (cut and paste) of the te. Contain additional genetic material besides transposase, irs (antibiotic resistant genes) Structure consistent with two iss close together in genome. One transposase may not be functional: inverted repeats are the signature of transposable elements, retrotransposons. Operate the same way as retroviruses (ex: hiv) Retroelement makes a copy and puts it somewhere else in the.
