MCB 3020 Lecture Notes - Lecture 3: Fluorescence Microscope, Microscope Slide, Sulfide

• School: University of Florida
• Department: Microbiology and Cell Science
• Course: MCB 3020
• Professor: Abdolkarim Asghari

Document Summary

Saturated fatty acids can cause arteries to get hard. Nucleotides bases/nucleic acids: dna or rna strands composed of nucleotides, dna, rna, carry blueprint of the cell, rna also serve as: Proteins (most abundant of all polymers) are made of amino acids. It folds based on properties of amino acids: structures of proteins (order of complexity), primary: peptide (covalent) bonds, secondary: helical structure, h bonds, tertiary: hydrophilic, van der waals, sulfide bridge. Factors affecting protein shape: ph, temperature, salt concentration, changing these variables alters the activity/function of a protein. If protei(cid:374)"s shape is altered so se(cid:448)erely that the protei(cid:374) (cid:374)o lo(cid:374)ger fu(cid:374)(cid:272)tio(cid:374)s, the protei(cid:374) is said to be denatured. Light microscopy: bright-field microscope: light goes from bottom, passes through specimen, dark-field microscope: dark background, specimen illuminated, phase-contrast microscope: can see outline of specimen. Uses uv light: fluorescence microscope: uses uv light and fluorescent dye. A diagnostic tool: electron microscopy, tem details of inside of cell.