BIOLOGY 172 Lecture Notes - Lecture 19: Replica Plating, Operon, Galactose
Document Summary
Gene expression: cells difer, structure, funcion, acivity, every cell has same dna, cells can difer in the funcion based on expressed genes consituive gene products are made coninuously regulated expression product made on demand c. d. Expression + informaion flow: transcripional control, focus on transcripional control, every genes must be transcribed, first step in expression, most important aspect of their gene expression, translaion control, post-translaional control. Lac operon example of prokaryoic expression: gene screening, mutagenic screen. If you mutate the gene necessary for metabolism, cell won"t metabolize. 1: can help determine more important genes for metabolism, random mutaions in the genome, replica plaing, grow same colony on two diferent growth condiions, all cells of each colony is geneically idenical. 3: stamped from one plate to the other, can compare growth on both plates. 3: promoter and operatore sequences are too close for both laci and rna.
