BIOLOGY 172 Lecture Notes - Lecture 31: Gametogenesis, Gynoecium, Petal
Document Summary
Gene over-expression to analyze expression: can engineer a gain-of-function mutation a. i. Is eyeless at the top of the eye developmental hierarchy? a. iii. Can distinguish between master regulator or other genes that affect eye growth a. iii. 2. Eyeless loss-of-function has no eyes: how to target over-expression b. i. Gal4 transcription factors (from yeast, do nothing without uas promoter sequence) must be paired for this to work b. iii. Germ-line transformation causes drosophila to carry this construct b. iii. 1. Gal4 protein binds to uas and expresses eyeless wherever this construct exists b. iii. 2. For this purpose, eyeless was over-expressed in the wing, can be done in wings: eyeless is master regulator c. i. Activate all the genes for eye development c. ii. Eye development was complete when expressed elsewhere: gene function can be conserved d. i. Short development period: differences b/w plant and animal development b. i. Plant embryonic structures take shape because of precise divisions b. iii. Nearly all plant organs are formed post-embryonically b. iii. 1.
