EEB 390 Lecture Notes - Lecture 16: Sanger Sequencing, Horizontal Gene Transfer, Human Genome Project

• School: U of M
• Department: Ecology and Evolutionary Biology
• Course: EEB 390
• Professor: Jeff Smith

Document Summary

Evolutionary genomics: the study of how the composition and structure of genomes have evolved and are evolving: new eld because of developments in sequencing techniques. Sequencing: 1976-1977: early sanger developments in the early 1970"s, maxam-gilbert in 1976, dangerous, complicated, slow, sanger in 1997, chain termination method, fast, fewer toxicity, simple. Pcr: 1985: replicate particular regions of genome which made it easier to ask particular question and sequence. Human genome project: 1990-2003: started with ncbi, plan: 13 billion over 15 years, celera corp started in "98, fist draft publish in 2013. This is so important because developments in next generation sequencing: whole genome you extracted and you would sequence the whole thing, cut into different chunk and sequence chunks and put them back together, whole sub eld of genome assembly. Evolutionary genomics: studies genome-wide patterns of evolution, function (content and composition, rearrangements (duplications, deletions, inversions, etc. , size (growth and reduction, patterns of selection.