MCDB 427 Lecture Notes - Lecture 11: Tata Box, Peptide, Tata-Binding Protein

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purplebeaver856

21 Dec 2016

School

U of M

Department

Molecular, Cellular and Developmental Biology

Course

MCDB 427

Professor

Janine Maddock

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Document Summary

Gtfs are needed for eukaryotic rnap to be able to initiate transcription. Gtfs assemble in the core promoter allowing rnap to get involved and initiate transcription. These are transcriptional factors that are used by rnap 2. They help recognize and initiate transcription at a promoter. There are 6 of these gtfs for rnap 2. How do the gtfs come to recognize the correct sequence and help to get rnap involved? (next slide) It is an emsa assay so it uses a labeled dna piece (this one is a promoter sequence that is strongly expressed from antovirus) and then various proteins are added to that labeled dna. If so, will they retart the mobility of that dna in a gel so instead of seeing the naked dna on the gel, we"ll see bands higher up. There were 5 protein complexes that were tested. Note: for lane 13, -d means that they included all the proteins except d.

Related Questions

As you may know, gene transcription begins with the assembly of the transcriptional machinery (TFIIA-H proteins) at the core promoter of genes. Where do you think core promoters are more likely to be located? Nucleosome DNA or Linker DNA?

Go ahead and formulate a hypothesis on the more likely location of core promoters of genes in DNA, and defend it with molecular biology arguments (in other words, why would one or the other be a preferred location for core promoters, based on what you know about nucleosome DNA and linker DNA?).

Then, I want you to imagine that you have access to a standard molecular genetics lab, and I want you to propose an experimental test of your hypothesis. You donât need to get too technical, just a description of how you would design an experiment that tests your hypothesis, indicating samples, treatments, and the measurements or readouts that that you plan to take. Your imaginary lab has the following equipment:

1) Antibodies for immunoprecipitation: these are molecules that can bind to several kinds of proteins, and allow you to physically pull them down (along with anything that is bound to them). In this case, imagine that you have access to antibodies that allow you to separate any proteins bound to DNA and isolate them along with any DNA bound to them!!

2)DNA sequencer: a machine that allows you to âreadâ the sequence of a given DNA sample.

3)Core promoter sequences: Information about what a typical core promoter sequence looks like.

So go ahead and design an experiment using any combination of one or more of the resources listed above, and do not forget to describe what results you expect to see, should your hypothesis be correct.

Your submission should be 200 words or fewer,

PLEASE use ONLY the equipment mentioned above.

cerisemacaw424

As you may know from previous courses, gene transcription begins with the assembly of the transcriptional machinery (TFIIA-H proteins) at the core promoter of genes. Where do you think core promoters are more likely to be located? Nucleosome DNA or Linker DNA?

2)DNA sequencer: a machine that allows you to âreadâ the sequence of a given DNA sample.

3)Core promoter sequences: Information about what a typical core promoter sequence looks like.

Your submission should be 200 words or fewer,

viridianmule62

MCDB 427 Lecture Notes - Lecture 11: Tata Box, Peptide, Tata-Binding Protein

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