CHEN 3701 Lecture Notes - Lecture 12: Lansoprazole, Plat, Paga
8 Oct 2015
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12:07 pm: an enzyme catalyzes the irreversible conversion of substrate to product following the reversible formation of an enzyme-substrate complex. The following data were obtained with 0. 5 enzyme. Note that identical data are plotted on the linear and semilog plots. 1000: on the semilog plot, sketch the expected data if the enzyme concentration were reduced to 0. 25 microm. Note important data points that aided your sketch. Km is unchanged the rate is halved everywhere: assuming pseudosteady state enzyme-substrate complex, calculate the first-order rate constant for dissociation of enzyme and substrate. Write a mechanism for the rxn scheme and derive all equations needed. Assume the association rate constant equals 5*105 m-1s-1: rate eqn, [es, mass balance on enzyme, assumption: pssa and plug in mass balance too. Use semilog plot b/c goes to higher substrate concentrations, where we have saturated the system w/ substrate.
